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KMID : 1098420160240030228
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Korean Journal of Medicinal Crop Science
2016 Volume.24 No. 3 p.228 ~ p.236
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Anti-Inflammatory Effect of Sedum takesimense Nakai Water Extract in RAW 264.7 Cells
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Jang Ji-Hun
Jung Ho-Kyung
Ko Jae-Hyung
Sim Mi-Ok
Woo Kyeong-Wan
Kim Tae-Muk
Lee Ki-Ho
An Byeong-Kwan
Cho Hyun-Woo
Cho Jung-Hee
Jung Won-Seok
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Abstract
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Background: Sedum takesimense Nakai has been used as folk medicine in Korea. The present study aimed to determine the biological activity of S. takesimense by investigating the anti-inflammatory effects of S. takesimense water extract (SKLC) on the lipopolysaccharide-induced inflammatory response in RAW 264.7 cells.
Methods and Results: Cytotoxicity of SKLC on RAW 264.7 cells was determinded by performing MTS assay was found to have no cytotoxic effect on RAW 264.7 cells at a concentration range of 62 - 500 ㎍/㎖. Further, pretreatment of SKLC inhibited lipopolysaccharide-induced nitric oxide (NO) production in a dose-dependent manner. To determined the inhibitory mechanisms of SKLC on inflammatory mediators, we assessed the inducible nitric oxide synthase (iNOS) and cyclooxygnease-2 (COX-2) pathways. The activities of these pathways were decreased in a dose-dependent manner by SKLC. The production of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β? and IL-6 were also reduced.
Conclusions: These results suggest that the down regulation of iNOS, COX-2, TNF-α, IL-1β? and IL-6 expression by SKLC are mediated by the down regulation of nuclear factor-κB (NF-κB) activity, a transcription factor necessary for pro-inflammatory mediators. This might be the mechanism underlying the anti-inflammatory effects of SKLC.
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KEYWORD
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Sedum takesimense Nakai, Anti-Inflammatory Effect, RAW 264.7
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